Abstract
Understanding the development of an organ requires knowledge of gene, protein, and metabolite expression in the specific cell types and tissues that comprise the organ. Fluorescence-activated cell sorting (FACS) is an efficient method to isolate specific cells of interest, and the information gained from this approach has been integral to plant developmental biology. The Benfey lab has developed this method to examine gene expression profiles of different cell types in the Arabidopsis root under both standard and stress conditions. In addition to gene expression, downstream applications of FACS include proteomic and metabolite analysis. This is a powerful method to examine biological functions of specific cell types and tissues with a systems biology approach.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Birnbaum, K., Shasha, D. E., Wang, J. Y., Jung, J. W., Lambert, G. M., Galbraith, D. W., et al. (2003) A gene expression map of the Arabidopsis root. Science 302, 1956–1960.
Brady, S. M., Orlando D. A., Lee, J. Y., Wang J. Y., Koch, J., Dinneny J. R., et al. (2007) A high-resolution root spatiotemporal map reveals dominant expression patterns. Science 318, 801–806.
Dinneny, J. R., Long, T. A., Wang, J. Y., Jung, J. W., Mace, D., Pointer, S., et al. (2008) Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320, 942–945.
Zhang, C., Barthelson, R. A., Lambert, G. M., and Galbraith, D. W. (2008) Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. Plant Physiol 147, 30–40.
Galbraith, D. W. and Birnbaum, K. (2006) Global studies of cell type-specific gene expression in plants. Annu Rev Plant Biol 57, 451–475.
Brandt, S. P. (2005) Microgenomics: Gene expression analysis at the tissue-specific and single-cell levels. J Exp Bot 56, 495–505.
Birnbaum, K., Jung, J. W., Wang, J. Y., Lambert, G. M., Hirst, J. A., Galbraith, D. W., et al. (2005) Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines. Nat Protoc 2, 615–619.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Iyer-Pascuzzi, A.S., Benfey, P.N. (2010). Fluorescence-Activated Cell Sorting in Plant Developmental Biology. In: Hennig, L., Köhler, C. (eds) Plant Developmental Biology. Methods in Molecular Biology, vol 655. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-765-5_21
Download citation
DOI: https://doi.org/10.1007/978-1-60761-765-5_21
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-764-8
Online ISBN: 978-1-60761-765-5
eBook Packages: Springer Protocols