Abstract
Light is one of the most important exogenous factors regulating plant development throughout the entire life cycle. Light is involved in the breaking of seed dormancy, the regulation of photomorphogenic seedling development, the adaptation of plant morphology toward spectral composition of incident light, and the transition to flowering. Plants have evolved with several photoreceptor families that sense UV-A, blue, red, and far-red light. Here, basal methods to measure light-regulated changes in plant morphology and pigment accumulation will be described. The methods include the determination of apical hook angle and cotyledon opening, the measurement of stem elongation, the determination of leaf surface area, the measurements that characterize light-controlled transition to flowering, and the determination of anthocyanin and chlorophyll accumulation. Furthermore, different light programs are listed that can be used to test for the functional involvement of separate light response modes controlling photomorphogenic plant development.
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References
Casal, J. J. and Sanchez, R. A. (1998) Phytochromes and seed germination. Seed Sci Res 8, 317–329.
Chen, M., Chory, J., and Fankhauser, C. (2004) Light signal transduction in higher plants. Annu Rev Genet 38, 81–117.
Franklin, K. A., Larner, V. S., and Whitelam, G. C. (2005) The signal transducing photoreceptors of plants. Int J Dev Biol 49, 653–664.
Yanosky, M. J. and Kay, S. A. (2003) Living by the calendar: How plants know when to flower. Nat Rev Mol Cell Biol 4, 265–275.
Rockwell, N. C., Su, Y. S., and Lagarias, J. C. (2006) Phytochrome structure and signaling mechanisms. Annu Rev Plant Biol 57, 837–858.
Mancinelli, A. L. (1994) The physiology of phytochrome action. In: Photomorphogenesis in Plants, 2nd Edition, pp. 51–59. Kendrick, R. E. and Kronenberg, G. H. M., eds. Kluwer, Dordrecht.
Rasband, W. S., Image, J., U. S. National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/, 1997–2008.
Schmidt, R. and Mohr, H. (1981) Time-dependent changes in the responsiveness to light of phytochrome-mediated anthocyanin synthesis. Plant Cell Environ 4, 433–437.
Moran, R. (1982) Formulae for determination of chlorophyllus pigments extracted with N,N-dimethylformamid. Plant Physiol 69, 1376–1381.
Moran, R. and Porath, D. (1980) Chlorophyll determination in intact tissues using N,N-dimethylformamid. Plant Physiol 65, 478–479.
Smeekens, S. (2000) Sugar-induced signal transduction in plants. Annu Rev Plant Physiol Plant Mol Biol 51, 49–81.
Zhou, Y.-C., Dieterle, M., and Kretsch, T. (2002) The negatively acting factors EID1 and SPA1 have distinct functions in phytochrome A-specific light signaling. Plant Physiol 128, 1098–1108.
Acknowledgments
This work was supported by the DFG grant ‘Analysis of phytochrome A-dependent light signalling in Arabidopsis thaliana’ (KR2020/2–4).
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Kretsch, T. (2010). Phenotypic Characterization of Photomorphogenic Responses During Plant Development. In: Hennig, L., Köhler, C. (eds) Plant Developmental Biology. Methods in Molecular Biology, vol 655. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-765-5_13
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DOI: https://doi.org/10.1007/978-1-60761-765-5_13
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Publisher Name: Humana Press, Totowa, NJ
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