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Crystallography of Membrane Proteins: From Crystallization to Structure

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Membrane Protein Structure Determination

Part of the book series: Methods in Molecular Biology ((MIMB,volume 654))

Abstract

Although crystallographic studies of membrane proteins have progressed in the last 5 years, the field still remains challenging with several severe bottlenecks. The chapter focuses on the crystallization and describes two approaches, the classical vapor diffusion method and the more recent use of lipidic phases. General aspects on the crystallization principles as well as more practical details are given. In a more synthetic way, the chapter also addresses how structures are solved by X-ray crystallography, and highlights aspects that are specific to membrane proteins.

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Authors and Affiliations

  1. European Molecular Biology Laboratory, Grenoble Outstation, B.P. 181, Grenoble, France

    Aurélien Deniaud

  2. Institut de Biologie Structurale, CEA-CNRS and Université Joseph Fourier, Grenoble, France

    Ekaterina Moiseeva, Valentin Gordeliy & Eva Pebay-Peyroula

Authors
  1. Aurélien Deniaud
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  2. Ekaterina Moiseeva
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  3. Valentin Gordeliy
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  4. Eva Pebay-Peyroula
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Corresponding author

Correspondence to Eva Pebay-Peyroula .

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Editors and Affiliations

  1. , Université Paris Diderot - Paris 7, INSERM U773/CRB3, rue Henri Huchard 16, Paris, 75018, France

    Jean-Jacques Lacapère

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Deniaud, A., Moiseeva, E., Gordeliy, V., Pebay-Peyroula, E. (2010). Crystallography of Membrane Proteins: From Crystallization to Structure. In: Lacapère, JJ. (eds) Membrane Protein Structure Determination. Methods in Molecular Biology, vol 654. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-762-4_5

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  • DOI: https://doi.org/10.1007/978-1-60761-762-4_5

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  • Publisher Name: Humana Press, Totowa, NJ

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