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Membrane Protein Structure Determination pp 303–319Cite as

What Can We Learn from a Small Regulatory Membrane Protein?

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 654))

Abstract

This chapter reviews the molecular biology, biochemical, and NMR methods that we used to study the structural dynamics, membrane topology, and interaction of phospholamban (PLN), a small regulatory membrane protein involved in the regulation of the sarcoplasmic reticulum Ca-ATPase (SERCA). In particular, we show the progression of our research from the initial hypotheses toward understanding the molecular mechanisms of SERCA’s regulation, including the effects of PLN oligomerization and posttranslational phosphorylation. Finally, we show how the knowledge of the molecular mechanism of the structural dynamics and topology of free and bound proteins can lead to the rational design of PLN analogs for possible use in gene therapy.

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Acknowledgment

This work was supported by grants to GV from the National Institutes of Health (GM64742, HL80081, GM072701) and NJT (AHA 0515491Z). PISEMA spectra were acquired at the NHMFL, Tallahassee, FL (DMR-0084173). NMR instrumentation at the University of Minnesota High Field NMR Center was funded by the National Science Foundation (BIR-961477) and the University of Minnesota Medical School.

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Authors and Affiliations

  1. Department of Chemistry and Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA

    Gianluigi Veglia

  2. Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA

    Kim N. Ha, Raffaello Verardi & Nathaniel J. Traaseth

  3. Department of Chemistry, University of Minnesota, Minneapolis, MN, USA

    Lei Shi

Authors
  1. Gianluigi Veglia
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  2. Kim N. Ha
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  3. Lei Shi
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  4. Raffaello Verardi
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  5. Nathaniel J. Traaseth
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Corresponding author

Correspondence to Gianluigi Veglia .

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Editors and Affiliations

  1. , Université Paris Diderot - Paris 7, INSERM U773/CRB3, rue Henri Huchard 16, Paris, 75018, France

    Jean-Jacques Lacapère

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Veglia, G., Ha, K.N., Shi, L., Verardi, R., Traaseth, N.J. (2010). What Can We Learn from a Small Regulatory Membrane Protein?. In: Lacapère, JJ. (eds) Membrane Protein Structure Determination. Methods in Molecular Biology, vol 654. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-762-4_16

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  • DOI: https://doi.org/10.1007/978-1-60761-762-4_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-761-7

  • Online ISBN: 978-1-60761-762-4

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