Abstract
Alterations in the genome that lead to changes in DNA sequence copy number are a characteristic of Glioblastomas (GBs). Microarray-based comparative genomic hybridization (array-CGH) is a high-throughput technology that allows the hybridization of genomic DNA onto conventional cDNA microarrays, normally used in expression profiling, to analyze genomic copy number imbalances. In this way, thousands of genes can be reviewed in a high resolution analysis to define amplicons and to identify? candidate genes showing recurrent genomic copy number changes in GB tumors.
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Acknowledgments
The author would like to thank Elena Gómez from the Virgen de la Salud Hospital for her technical assistance. This work was partially supported by grants FIS CA07/00119, FIS 03/0727, FIS PI070662 and INT 07/028 from the Fondo de Investigaciones Sanitarias; and FISCAM PI-2006/29 from the Consejeria de Sanidad de la Junta de Comunidades de Castilla-La Mancha.
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Ruano, Y., Mollejo, M., de Lope, A.R., Hernández-Moneo, J.L., Martínez, P., Meléndez, B. (2010). Microarray-Based Comparative Genomic Hybridization (Array-CGH) as a Useful Tool for Identifying Genes Involved in Glioblastoma (GB). In: Webb, M. (eds) Cancer Susceptibility. Methods in Molecular Biology, vol 653. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-759-4_3
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DOI: https://doi.org/10.1007/978-1-60761-759-4_3
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