Skip to main content
SpringerLink
Log in

Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS

  • Protocol
  • First Online:
Book cover Mass Spectrometry Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 656))

Abstract

Imaging mass spectrometry (IMS) allows the direct investigation of both the identity and the spatial distribution of the entire molecular content directly in tissue sections, single cells, and many other biological surfaces. We describe here the steps required to retrieve the molecular information from tissue sections using matrix-enhanced (ME) and metal-assisted (MetA) secondary ion mass spectrometry (SIMS). Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight (ToF) SIMS of tissues and cells. High-resolution images of cholesterol and other membrane components can be obtained for single neuroblastoma cells and reveal subcellular details. Alternatively, in ME-SIMS, 2,5-dihydroxybenzoic acid electrosprayed on neuroblastoma cells allows intact molecular ion imaging of phosphatidylcholine (PC) and sphingomyelin (SM) at the cellular level.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Vickerman, J. C., Briggs, D. (Eds.) (2001) ToF-SIMS: Surface Analysis by Mass Spectrometry, IM Publications and SurfaceSpectra Limited, Chichester.

    Google Scholar 

  2. Todd, P. J., McMahon, J. M., Short, R. T., McCandlish, C. A. (1997) Organic SIMS of biologic tissue. Anal Chem, 69, 529A–535A.

    Article  PubMed  CAS  Google Scholar 

  3. Delcorte, A., Garrison, B. J. (2000) High yield events of molecular emission induced by kiloelectronvolt particle bombardment. J Phys ChemB,104, 6785–6800.

    CAS  Google Scholar 

  4. Chandra, S., Morrison, G. H. (1995) Imaging ion and molecular-transport at subcellular resolution by secondary-ion mass-spectrometry. Int J Mass Spectrom Ion Process, 143, 161–176.

    Article  CAS  Google Scholar 

  5. Chandra, S., Smith, D. R., Morrison, G. H. (2000) Subcellular imaging by dynamic SIMS ion microscopy. Anal Chem, 72, 104A–114A.

    Article  PubMed  CAS  Google Scholar 

  6. Strick, R., Strissel, P. L., Gavrilov, K., Levi-Setti, R. (2001) Cation–chromatin binding as shown by ion microscopy is essential for the structural integrity of chromosomes. J Cell Biol, 155, 899–910.

    Article  PubMed  CAS  Google Scholar 

  7. Colliver, T. L., Brummel, C. L., Pacholski, M. L., Swanek, F. D., Ewing, A. G., Winograd, N. (1997) Atomic and molecular imaging at the single-cell level with TOF-SIMS. Anal Chem, 69, 2225–2231.

    Article  PubMed  CAS  Google Scholar 

  8. Pacholski, M. L., Cannon, D. M., Ewing, A. G., Winograd, N. (1998) Static time-of-flight secondary ion mass spectrometry imaging of freeze-fractured, frozen-hydrated biological membranes. Rapid Commun Mass Spectrom, 12, 1232.

    Article  PubMed  CAS  Google Scholar 

  9. Todd, P. J., Schaaff, T. G., Chaurand, P., Caprioli, R. M. (2001) Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization. J Mass Spectrom 36, 355–369.

    Article  PubMed  CAS  Google Scholar 

  10. Altelaar, A. F. M., van Minnen, J., Jimenez, C. R., Heeren, R. M. A., Piersma, S. R. (2005) Direct molecular imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by mass spectrometry. Anal Chem, 77, 735–741.

    Article  PubMed  CAS  Google Scholar 

  11. Luxembourg, S. L., McDonnell, L. A., Duursma, M. C., Guo, X. H., Heeren, R. M. A. (2003) Effect of local matrix crystal variations in matrix-assisted ionization techniques for mass spectrometry. Anal Chem, 75, 2333–2341.

    Article  PubMed  CAS  Google Scholar 

  12. McDonnell, L. A., Piersma, S. R., Altelaar, A. F. M., Mize, T. H., Luxembourg, S. L., Verhaert, P., van Minnen, J., Heeren, R. M. A. (2005) Subcellular imaging mass spectrometry of brain tissue. J Mass Spectrom, 40, 160–168.

    Article  PubMed  CAS  Google Scholar 

  13. Wittmaack, K., Szymczak, W., Hoheisel, G., Tuszynski, W. (2000) Time-of-flight secondary ion mass spectrometry of matrix-diluted oligo- and polypeptides bombarded with slow and fast projectiles: positive and negative matrix and analyte ion yields, background signals, and sample aging. J Am Soc Mass Spectrom, 11, 553–563.

    Article  PubMed  CAS  Google Scholar 

  14. Wu, K. J., Odom, R. W. (1996) Matrix-enhanced secondary ion mass spectrometry: a method for molecular analysis of solid surfaces. Anal Chem, 68, 873–882.

    Article  PubMed  CAS  Google Scholar 

  15. Nygren, H., Johansson, B. R., Malmberg, P. (2004) Bioimaging TOF-SIMS of tissues by gold ion bombardment of a silver-coated thin section. Microsc Res Tech, 65, 282–286.

    Article  PubMed  CAS  Google Scholar 

  16. Adriaensen, L., Vangaever, F., Gijbels, R. (2004) Metal-assisted secondary ion mass spectrometry: influence of Ag and Au deposition on molecular ion yields. Anal Chem, 76, 6777–6785.

    Article  PubMed  CAS  Google Scholar 

  17. Delcorte, A., Bertrand, P. (2004) Interest of Silver and Gold Metallization for Molecular SIMS and SIMS Imaging, 250–255, Elsevier Science, London.

    Google Scholar 

  18. Delcorte, A., Bour, J., Aubriet, F., Muller, J. F., Bertrand, P. (2003) Sample metallization for performance improvement in desorption/ionization of kilodalton molecules: quantitative evaluation, imaging secondary ion MS, and laser ablation. Anal Chem, 75, 6875–6885.

    Article  PubMed  CAS  Google Scholar 

  19. Delcorte, A., Medard, N., Bertrand, P. (2002) Organic secondary ion mass spectrometry: sensitivity enhancement by gold deposition. Anal Chem, 74, 4955–4968.

    Article  PubMed  CAS  Google Scholar 

  20. Keune, K., Boon, J. J. (2004) Enhancement of the static SIMS secondary ion yields of lipid moieties by ultrathin gold coating of aged oil paint surfaces. Surf Interface Anal, 36, 1620–1628.

    Article  CAS  Google Scholar 

  21. Nguyen, T. C., Ward, D. W., Townes, J. A., White, A. K., Krantzman, K. D., Garrison, B. J. (2000) A theoretical investigation of the yield-to-damage enhancement with polyatomic projectiles in organic SIMS. J Phys Chem B, 104, 8221–8228.

    Article  CAS  Google Scholar 

  22. Sjovall, P., Lausmaa, J., Johansson, B. (2004) Mass spectrometric imaging of lipids in brain tissue. Anal Chem, 76, 4271–4278.

    Article  PubMed  Google Scholar 

  23. Todd, P. J., McMahon, J. M., McCandlish, C. A. (2004) Secondary ion images of the developing rat brain. J Am Soc Mass Spectrom, 15, 1116–1122.

    Article  PubMed  CAS  Google Scholar 

  24. Touboul, D., Halgand, F., Brunelle, A., Kersting, R., Tallarek, E., Hagenhoff, B., Laprevote, O. (2004) Tissue molecular ion imaging by gold cluster ion bombardment. Anal Chem, 76, 1550–1559.

    Article  PubMed  CAS  Google Scholar 

  25. Townes, J. A., White, A. K., Wiggins, E. N., Krantzman, K. D., Garrison, B. J., Winograd, N. (1999) Mechanism for increased yield with SF5+ projectiles in organic SIMS: the substrate effect. J Phys Chem A, 103, 4587–4589.

    Article  CAS  Google Scholar 

  26. Weibel, D., Wong, S., Lockyer, N., Blenkinsopp, P., Hill, R., Vickerman, J. C. (2003) A C-60 primary ion beam system for time of flight secondary ion mass spectrometry: its development and secondary ion yield characteristics. Anal Chem, 75, 1754–1764.

    Article  PubMed  CAS  Google Scholar 

  27. Altelaar, A. F. M., Klinkert, I., Jalink, K., de Lange, R. P. J., Adan, R. A. H., Heeren, R. M. A., Piersma, S. R. (2006) Gold-enhanced biomolecular surface imaging of cells and tissue by SIMS and MALDI mass spectrometry. Anal Chem, 78, 734–742.

    Article  PubMed  CAS  Google Scholar 

  28. Broersen, A., van Liere, R., Altelaar, A. F. M., Heeren, R. M. A., McDonnell, L. A. (2008) Automated, feature-based image alignment for high-resolution imaging mass spectrometry of large biological samples. J Am Soc Mass Spectrom, 19, 823–832.

    Article  PubMed  CAS  Google Scholar 

  29. Svensson, M., Skold, K., Svenningsson, P., Andren, P. E. (2003) Peptidomics-based discovery of novel neuropeptides. J Proteome Res, 2, 213–219.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands

    A.F. Maarten Altelaar

  2. OncoProteomics Laboratory, Department of Medical Oncology, VUmc-Cancer Center Amsterdam, VU University Medical Center Amsterdam, Amsterdam, The Netherlands

    Sander R. Piersma

Authors
  1. A.F. Maarten Altelaar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sander R. Piersma
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. , Beckman Institute, Univ. of Illinois at Urbana-Champaign, N. Mathews Avenue 405, Urbana, 61801, Illinois, USA

    Stanislav S. Rubakhin

  2. , Department of Chemistry, Univ. of Illinois at Urbana-Champaign, South Mathews Avenue 63-5 600, Urbana, 61801, Illinois, USA

    Jonathan V. Sweedler

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Altelaar, A.M., Piersma, S.R. (2010). Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS. In: Rubakhin, S., Sweedler, J. (eds) Mass Spectrometry Imaging. Methods in Molecular Biology, vol 656. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-746-4_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-60761-746-4_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-745-7

  • Online ISBN: 978-1-60761-746-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation