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Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol

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Mass Spectrometry Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 656))

Abstract

Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging [MSI]) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging; however, the number of studies reporting imaging of small organic molecules has recently increased. IMS is an effective technique for the visualization of endogenous small metabolites, especially lipids, facilitated by the unique advantages of mass spectrometry-based molecular detection. Despite the promising capability of MALDI-IMS for imaging small metabolites, this technique still has several issues, especially in spatial resolution. One of the critical limitations of the spatial resolution of MALDI-IMS is the size of the organic matrix crystal and the analyte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is outlined. In addition, as an attractive alternative to MALDI-based IMS, we also present nanoparticle-based IMS that improves spatial resolution.

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Acknowledgments

The authors would like to thank Dr. S. Taira and Dr. H. Ageta for their advice and fruitful discussion. This work was supported by the SENTAN program of the Japan Science and Technology Agency.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, Japan

    Yuki Sugiura

  2. Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan

    Yuki Sugiura & Mitsutoshi Setou

  3. Department of Molecular Anatomy, Hamamatsu University School of Medicine, Shizuoka, Japan

    Mitsutoshi Setou

Authors
  1. Yuki Sugiura
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  2. Mitsutoshi Setou
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Editors and Affiliations

  1. , Beckman Institute, Univ. of Illinois at Urbana-Champaign, N. Mathews Avenue 405, Urbana, 61801, Illinois, USA

    Stanislav S. Rubakhin

  2. , Department of Chemistry, Univ. of Illinois at Urbana-Champaign, South Mathews Avenue 63-5 600, Urbana, 61801, Illinois, USA

    Jonathan V. Sweedler

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Sugiura, Y., Setou, M. (2010). Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol. In: Rubakhin, S., Sweedler, J. (eds) Mass Spectrometry Imaging. Methods in Molecular Biology, vol 656. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-746-4_10

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  • DOI: https://doi.org/10.1007/978-1-60761-746-4_10

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  • Publisher Name: Humana Press, Totowa, NJ

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