Abstract
In the age of systems biology, biologists seek to quantify the absolute number of molecules in experimentally treated samples. Immunoblotting remains a technique of choice for assessing the relative differences between the protein levels in different samples. Here we discuss how to exploit immunoblotting for estimating the number of Smad transcription factor molecules per cell. We focus on describing the calculations needed to analyze the data. Our methods are generally applicable to the quantification of other cellular proteins.
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Acknowledgments
We thank Scott Dixon, Dana Ungermannova, and Meredith Brown for critical reading of the manuscript. This work was supported by a National Institutes of Health grant (GM083172) to X. L.
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Clarke, D.C., Liu, X. (2010). Measuring the Absolute Abundance of the Smad Transcription Factors Using Quantitative Immunoblotting. In: Higgins, P. (eds) Transcription Factors. Methods in Molecular Biology, vol 647. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-738-9_22
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DOI: https://doi.org/10.1007/978-1-60761-738-9_22
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60761-738-9
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