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A Murine Autoimmune Model of Rheumatoid Arthritis and Systemic Lupus Erythematosus Associated with Deregulated Production of IL-17 and IL-21

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Autoimmunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 900))

Abstract

T-helper cell 17 (Th17) cells play an important role in the pathogenesis of many autoimmune disorders including Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE). In this chapter we describe a murine model where deregulated production of IL-17 and IL-21 can lead to either lupus-like disease or RA-like symptoms depending on the genetic background. We delineate the key techniques that can be used to dissect the mechanisms responsible for the pathogenesis of these diseases at both a cellular and molecular level including in vitro Th17 cell differentiation, chromatin immunoprecipitation assays, and retroviral transduction experiments. We also describe the methodologies that can be utilized to monitor the classic clinical findings of RA and SLE in murine models. Given the broad involvement of deregulated production of IL-17 and IL-21 in autoimmunity, many of these techniques could also be valuable for the investigation of these pathways in murine models of other autoimmune diseases.

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Acknowledgments

This work was supported by the Alliance for Lupus Research, the Mary Kirkland Center for Lupus Research, and the NIH (HL62215 and AI076474 to A.P.).

Author information

Authors and Affiliations

  1. Autoimmunity & Inflammation Program, Hospital for Special Surgery, New York, NY, USA

    Partha S. Biswas & Alessandra B. Pernis

  2. Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, New York, NY, USA

    Kyuho Kang & Alessandra B. Pernis

  3. Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, NY, USA

    Sanjay Gupta

  4. Department of Pathology and Cell Biology, Columbia University Medical Center and New York Presbyterian Hospital, New York, NY, USA

    Govind Bhagat

Authors
  1. Partha S. Biswas
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  2. Kyuho Kang
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  3. Sanjay Gupta
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  4. Govind Bhagat
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  5. Alessandra B. Pernis
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Corresponding author

Correspondence to Alessandra B. Pernis .

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Editors and Affiliations

  1. College of Medicine, Division of Rheumatology, State University of New York, UMU, East Adams St. 750, Syracuse, 13210, New York, USA

    Andras Perl

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Biswas, P.S., Kang, K., Gupta, S., Bhagat, G., Pernis, A.B. (2012). A Murine Autoimmune Model of Rheumatoid Arthritis and Systemic Lupus Erythematosus Associated with Deregulated Production of IL-17 and IL-21. In: Perl, A. (eds) Autoimmunity. Methods in Molecular Biology, vol 900. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-720-4_11

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  • DOI: https://doi.org/10.1007/978-1-60761-720-4_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-719-8

  • Online ISBN: 978-1-60761-720-4

  • eBook Packages: Springer Protocols

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