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Analysis of Renal Mononuclear Phagocytes in Murine Models of SLE

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Autoimmunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 900))

Abstract

In this chapter we present methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans. Using magnetic beads followed by flow cytometric sorting, pure populations of cells are obtained that are functional in a variety of assays. Sufficient numbers of cells are obtained for genomic characterization. An analysis of the function of these cells should lead to a better understanding of the inflammatory processes that cause renal impairment in SLE and other renal inflammatory diseases.

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Authors and Affiliations

  1. Center for Autoimmunity and Musculoskeletal Diseases, Feinstein Institute for Medical Research, Manhasset, New York, NY, USA

    Ramalingam Bethunaickan

  2. Center for Autoimmunity and Musculoskeletal Diseases, Feinstein Institute for Medical Research, Manhasset, NY, USA

    Ranjit Sahu & Anne Davidson

Authors
  1. Ramalingam Bethunaickan
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  2. Ranjit Sahu
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  3. Anne Davidson
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Corresponding author

Correspondence to Anne Davidson .

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Editors and Affiliations

  1. College of Medicine, Division of Rheumatology, State University of New York, UMU, East Adams St. 750, Syracuse, 13210, New York, USA

    Andras Perl

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Bethunaickan, R., Sahu, R., Davidson, A. (2012). Analysis of Renal Mononuclear Phagocytes in Murine Models of SLE. In: Perl, A. (eds) Autoimmunity. Methods in Molecular Biology, vol 900. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-720-4_10

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  • DOI: https://doi.org/10.1007/978-1-60761-720-4_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-719-8

  • Online ISBN: 978-1-60761-720-4

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