Abstract
Urine is a noninvasive sample that is ideal for screening, because it is easy to collect, cost-effective, and can provide a wealth of information on a patient’s health status. We provide a brief discussion on the anatomy and physiology of the kidney, a concise overview on B and T cells as key mediators of the immune system, and then delve into the various B-cell neoplasms. This discussion details Waldenstrom’s macroglobulinemia, plasmacytoma, heavy chain disease, amyloidosis, and multiple myeloma. Of primary clinical importance from a technical perspective, two commonly applied techniques for the separation and characterization of urine proteins include urine protein electrophoresis and urine protein immunofixation. Procedural details for both techniques are provided herein.
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Additional Reading
Sirohi, B., Powles, R. (2004) Multiple myeloma. Lancet 363:875–887.
Rosenfeld, L. (1987) Henry Bence Jones (1813–1873): the best “chemical doctor” in London. Clin Chem 33:1687–1692.
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Olivieri, B., Rai, A.J. (2010). A Primer on Clinical Applications and Assays Using Urine: Focus on Analysis of Plasma Cell Dyscrasias Using Automated Electrophoresis and Immunofixation. In: Rai, A. (eds) The Urinary Proteome. Methods in Molecular Biology, vol 641. Humana Press. https://doi.org/10.1007/978-1-60761-711-2_2
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DOI: https://doi.org/10.1007/978-1-60761-711-2_2
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Online ISBN: 978-1-60761-711-2
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