Abstract
As a novel potential therapeutic strategy for cardiac disease, cell transplantation therapy has been extensively investigated in experimental studies and clinical trials. Although encouraging results have been demonstrated, a number of critical questions still remain to be answered. For example, what kind of stem cell and how many cells should be used; what is the best time for cell transplantation after acute myocardial infarction; which delivery approach is better, intravenous injection or direct intramyocardial injection? Transplantation of cells derived from human tissues into experimental animals may elicit an immune rejection. Immunodeficient nude rats provide a useful myocardial infarction model for cell transplantation therapy studies. We introduce our detailed methods of direct intramyocardial injection of immature heart cells and stem cells into the myocardial infarction region of rats and nude rats. Careful maintenance under aseptic conditions and proper surgical technique are essential to improve the survival of immunodeficient rats after surgery.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dai W, Hale SL, Kloner RA. (2005) Stem cell transplantation for the treatment of myocardial infarction. Transpl Immunol. 15(2):91–7.
Dai W, Kloner RA. (2006) Myocardial regeneration by embryonic stem cell transplantation: present and future trends. Expert Rev Cardiovasc Ther. 4(3):375–83.
Dai W, Kloner RA. Mesenchymal stem cell therapy for the injured heart. In: Wollert KC, Field LJ, editors. Rebuilding the infarcted heart. London, UK: Informa Healthcare; 2007, p. 55–72.
Dow J, Simkhovich BZ, Kedes L, Kloner RA. (2005) Washout of transplanted cells from the heart: a potential new hurdle for cell transplantation therapy. Cardiovasc Res. 67(2):301–7.
Dai W, Field LJ, Rubart M, Reuter S, Hale SL, Zweigerdt R, Graichen RE, Kay GL, Jyrala AJ, Colman A, Davidson BP, Pera M, Kloner RA. (2007) Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts. J Mol Cell Cardiol. 43(4):504–16.
Hochman JS, Choo H. (1987) Limitation of myocardial infarct expansion by reperfusion independent of myocardial salvage. Circulation. 75(1):299–306.
Muller-Ehmsen J, Peterson KL, Kedes L, Whittaker P, Dow JS, Long TI, Laird PW, Kloner RA. (2002) Rebuilding a damaged heart: long-term survival of transplanted neonatal rat cardiomyocytes after myocardial infarction and effect on cardiac function. Circulation. 105(14):1720–6.
Acknowledgment
This work was supported in part by grant from the National Institutes of Health (R01-HL073709), the Los Angeles Thoracic and Cardiovascular Foundation, and ES Cell International Pte Ltd in Singapore.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Dai, W., Kloner, R.A. (2010). Experimental Cell Transplantation Therapy in Rat Myocardial Infarction Model Including Nude Rat Preparation. In: Lee, R. (eds) Stem Cells for Myocardial Regeneration. Methods in Molecular Biology, vol 660. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-705-1_7
Download citation
DOI: https://doi.org/10.1007/978-1-60761-705-1_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-704-4
Online ISBN: 978-1-60761-705-1
eBook Packages: Springer Protocols