Abstract
Vascular regeneration with bone marrow (BM) stem/progenitor cells is one of the promising therapeutic strategies for myocardial repair in cardiovascular diseases. Endothelial progenitor cells (EPCs) have demonstrated the beneficial effects on ischemia-induced myocardial damage promoting angiogenesis in ischemic tissue. Isolation of EPCs from BM or peripheral blood is required for the cell-based therapeutic approach. Among a variety of EPC isolation methods, specifically in the case of experimental animal models, we have shown our recently developed mouse-cultured EPC isolation protocol, which is viable for obtaining enough number of viable cells for not only in vitro but also in vivo experiments, and the protocol for human CD34+ cell (EPC rich cell population) isolation from peripheral blood and characterization by fluorescence-activated sorting (FACS) system.
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The author would like to thank Miki Horii and Ayumi Yokoyama for technical assistance.
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Ii, M. (2010). Bone Marrow-Derived Endothelial Progenitor Cells: Isolation and Characterization for Myocardial Repair. In: Lee, R. (eds) Stem Cells for Myocardial Regeneration. Methods in Molecular Biology, vol 660. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-705-1_2
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DOI: https://doi.org/10.1007/978-1-60761-705-1_2
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60761-705-1
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