Abstract
Mounting evidence suggests the regenerative potential of the mammalian heart. Nevertheless, the contribution of endogenous stem or precursor cells to adult cardiac regeneration upon myocardial injuries remains unclear. We hereby describe a genetic fate-mapping approach to study adult cardiomyocyte replenishment after myocardial injury. Using double transgenic MerCreMer–ZEG mice, the fate of adult cardiomyocytes can be tracked by the expression of green fluorescence protein (GFP) specifically induced in cardiomyocytes. Upon experimental myocardial infarction, a reduction in GFP expression in the myocardium is observed, indicating the refreshment of cardiomyocytes by endogenous stem or precursor cells.
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Acknowledgments
This work is supported by grants from the National Science Council (NSC 97IR082), the National Heath Research Institutes (NHRI EX97-9722SI) and the NCKU Landmark and Integrative Research Projects (NCKU 97I006).
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Chan, S.SK. et al. (2010). Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishment After Myocardial Injury. In: Lee, R. (eds) Stem Cells for Myocardial Regeneration. Methods in Molecular Biology, vol 660. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-705-1_13
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DOI: https://doi.org/10.1007/978-1-60761-705-1_13
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