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Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants

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Book cover Plant Stress Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 639))

Abstract

Glyoxalase system consists of two enzymes glyoxalase I (Gly I) and glyoxalase II (Gly II). Gly I detoxifies methylglyoxal (MG), a cytotoxic byproduct of glycolysis, to S-lactoylglutathione (SLG) where it uses one molecule of reduced glutathione. Subsequently, SLG is converted to lactate by Gly II and one molecule of reduced glutathione is recycled back into the system. The level of MG, which is produced ubiquitously in all living organisms, is enhanced upon exposure to different abiotic stresses in plants. Overexpression of glyoxalase pathway genes in transgenic plants has been found to keep a check on the MG level under stress conditions, regulate glutathione homeostasis, and the transgenic plants are able to survive and grow under various abiotic stresses.

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Acknowledgment

This work is supported by internal grants of ICGEB, New Delhi, and Department of Biotechnology, Government of India.

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Authors and Affiliations

  1. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India

    Ananda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek & Sudhir K. Sopory

Authors
  1. Ananda Mustafiz
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  2. Khirod K. Sahoo
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  3. Sneh L. Singla-Pareek
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  4. Sudhir K. Sopory
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Editors and Affiliations

  1. 246 NRC-D, Department of Biochemistry and Molecular, Oklahoma State University, Stillwater, 74078, Oklahoma, USA

    Ramanjulu Sunkar

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© 2010 Humana Press

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Mustafiz, A., Sahoo, K.K., Singla-Pareek, S.L., Sopory, S.K. (2010). Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 639. Humana Press. https://doi.org/10.1007/978-1-60761-702-0_6

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  • DOI: https://doi.org/10.1007/978-1-60761-702-0_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-701-3

  • Online ISBN: 978-1-60761-702-0

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