Abstract
The activity of most cellular processes is sensitive to pH. Cells therefore tightly control cytosol pH within narrow bounds. Measurement of cytosolic pH is of interest in studying many processes, including pH regulatory transport proteins. Key approaches that have been used to determine intracellular pH include pH-sensitive microelectrodes, nuclear magnetic resonance, and pH-sensitive fluorescent proteins. Here we review these approaches while providing details on the use of pH-sensitive fluorescent dyes to measure cytosolic pH.
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Acknowledgments
We would like to thank Dr. Bernardo Alvarez and Deborah Sterling for discussion and critical review of the chapter and the Academic Press and Molecular Probes, Inc., for permission to reprint figures from previous publications.
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Loiselle, F.B., Casey, J.R. (2010). Measurement of Intracellular pH. In: Yan, Q. (eds) Membrane Transporters in Drug Discovery and Development. Methods in Molecular Biology, vol 637. Humana Press. https://doi.org/10.1007/978-1-60761-700-6_17
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DOI: https://doi.org/10.1007/978-1-60761-700-6_17
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