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Transposon Insertional Mutagenesis in Rice

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Book cover Plant Reverse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 678))

Abstract

Insertion mutants offer one of the direct ways to relate a gene to its function by employing forward or reverse genetics approaches. Both T-DNA and transposon insertional mutants are being produced in several crops, including rice, the first cereal with its complete genome sequenced. Transposons have several advantages over T-DNA including the ability to produce multiple independent insertion lines from individual starter lines, as well as producing revertants by remobilization. With our new gene constructs, and a two-component transposon iAc/Ds mutagenesis protocol, we have improved both gene trapping and screening efficiencies in rice.

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Author information

Authors and Affiliations

  1. CSIRO Plant Industry, Canberra, ACT, Australia

    Narayana M. Upadhyaya & Qian-Hao Zhu

  2. University of Agricultural Sciences, Dharwad, Karnataka, India

    Ramesh S. Bhat

Authors
  1. Narayana M. Upadhyaya
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  2. Qian-Hao Zhu
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  3. Ramesh S. Bhat
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Editor information

Editors and Affiliations

  1. , Virginia Bioinformatics Institute, Virginia Tech, Washington Street, Blacksburg, 24061, Maryland, USA

    Andy Pereira

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Upadhyaya, N.M., Zhu, QH., Bhat, R.S. (2011). Transposon Insertional Mutagenesis in Rice. In: Pereira, A. (eds) Plant Reverse Genetics. Methods in Molecular Biology, vol 678. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-682-5_12

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  • DOI: https://doi.org/10.1007/978-1-60761-682-5_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-681-8

  • Online ISBN: 978-1-60761-682-5

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