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Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors

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Book cover Surface Plasmon Resonance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 627))

Abstract

Surface plasmon resonance has become one of the most important techniques for studying bimolecular interactions. Most of the researchers are using it to study protein–protein interactions, but in recent years membrane model systems have also become available and this makes it possible to study protein–membrane interactions as well. In this review chapter we describe possible ways to prepare lipid membrane surfaces on various sensor chips and some of the experimental considerations one has to take into account when performing such experiments.

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Acknowledgments

We would like to thank the Slovenian Research Agency for support and Vesna Hodnik for critically reading the manuscript.

Author information

Authors and Affiliations

  1. Department of Biology, Biotechnical Faculty, University of Ljubljana; Institute “Jožef Stefan”, Ljubljana, Slovenia

    Mojca Podlesnik Beseničar

  2. Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    Gregor Anderluh

Authors
  1. Mojca Podlesnik Beseničar
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  2. Gregor Anderluh
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Editor information

Editors and Affiliations

  1. Dept. Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, Utrecht, 3585 CA, Netherlands

    Nico J. Mol

  2. Dept. Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, Utrecht, 3585 CA, Netherlands

    Marcel J. E. Fischer

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Beseničar, M.P., Anderluh, G. (2010). Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors. In: Mol, N., Fischer, M. (eds) Surface Plasmon Resonance. Methods in Molecular Biology, vol 627. Humana Press. https://doi.org/10.1007/978-1-60761-670-2_12

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  • DOI: https://doi.org/10.1007/978-1-60761-670-2_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-669-6

  • Online ISBN: 978-1-60761-670-2

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