PCR/LDR/Universal Array Platforms for the Diagnosis of Infectious Disease

  • Maneesh PingleEmail author
  • Mark Rundell
  • Sanchita Das
  • Linnie M. Golightly
  • Francis Barany
Part of the Methods in Molecular Biology book series (MIMB, volume 632)


Infectious diseases account for between 14 and 17 million deaths worldwide each year. Accurate and rapid diagnosis of bacterial, fungal, viral, and parasitic infections is therefore essential to reduce the morbidity and mortality associated with these diseases. Classical microbiological and serological methods have long served as the gold standard for diagnosis but are increasingly being replaced by molecular diagnostic methods that demonstrate increased sensitivity and specificity and provide an identification of the etiologic agent in a shorter period of time. PCR/LDR coupled with universal array detection provides a highly sensitive and specific platform for the detection and identification of bacterial and viral infections.

Key words

Ligase detection reaction Microarray Multiplexed detection Bacterial identification Viral identification 



Support for this work was provided by the National Cancer Institute (P01-CA65930) and the National Institute of Allergy and Infectious Diseases (UC1-AI062579).


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Maneesh Pingle
    • 1
    Email author
  • Mark Rundell
    • 1
  • Sanchita Das
    • 1
  • Linnie M. Golightly
    • 1
  • Francis Barany
    • 1
  1. 1.Department of MicrobiologyWeill Medical College, Cornell UniversityNew YorkUSA

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