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Determination of Alternate Splicing Events Using the Affymetrix Exon 1.0 ST Arrays

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Microarray Methods for Drug Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 632))

Abstract

Alternative splicing plays an important role in regulation of normal cellular function. Alternative splicing of pre-mRNA leads to the diversity of downstream protein products in the cell. The Affymetrix Exon arrays allow for a high throughput evaluation of the differences in spliced mRNA expressed in a biological system. In this study, we describe a method using this technology to study the generation of alternative mRNA transcripts in breast cancer cells that differ in the levels of a particular integrin, α3β1.

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References

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Author information

Authors and Affiliations

  1. Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY, USA

    Sita Subbaram, C. Michael DiPersio & Sridar V. Chittur

  2. Department of Biomedical Sciences, Center for Functional Genomics, School of Public Health, University at Albany-SUNY, Rensselaer, NY, USA

    Marcy Kuentzel & David Frank

Authors
  1. Sita Subbaram
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  2. Marcy Kuentzel
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  3. David Frank
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  4. C. Michael DiPersio
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  5. Sridar V. Chittur
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Corresponding author

Correspondence to Sridar V. Chittur .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences Schools of Public Health Centre for Functional Genomics, University at Albany-SUNY, Rensselaer, NY, USA

    Sridar V. Chittur Ph.D.

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Subbaram, S., Kuentzel, M., Frank, D., DiPersio, C.M., Chittur, S.V. (2010). Determination of Alternate Splicing Events Using the Affymetrix Exon 1.0 ST Arrays. In: Chittur, S.V. (eds) Microarray Methods for Drug Discovery. Methods in Molecular Biology, vol 632. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-663-4_4

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  • DOI: https://doi.org/10.1007/978-1-60761-663-4_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-662-7

  • Online ISBN: 978-1-60761-663-4

  • eBook Packages: Springer Protocols

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