Production and Application of Glycan Microarrays
Glycans are vital elements of living organisms and are involved in recognition, communication, cell growth and development, motility, and other significant processes. The interactions of glycans with the proteins that bind them provide valuable information about protein interaction and specificity. By printing glycans on microarrays, investigators are able to effectively determine the binding specificity of certain proteins with an extremely efficient and precise result. Such chips are performed by standard robotic microarray printing. Incubating the slides with various GBP-containing substances not only reveals clear receptor preferences of the proteins, but also detects minute differences in structure specificity.
Key wordsGlycan Carbohydrate Lectin Microarray Glycan-binding protein Chip
The authors would like to thank Ola Blixt, James Paulson, Nahid Razi, and Julia Hoffmann for all of their help and guidance. Andrew Hemingway and all of Schott/Nexterion for outstanding product support. Special thanks to the Consortium for Funtional Glycomics (CFG) for making everything possible.
- 1.Blixt O, Head S, Mondala T, Scanlan C, Huflejt ME, Alvarez R, Bryan MC, Fazio F, Calarese D, Stevens J, Razi N, Stevens DJ, Skehel JJ, van Die I, Burton DR, Wilson IA, Cummings R, Bovin N, Wong C-H, Paulson JC (2004) Printed covalent glycan array for ligand profiling of diverse glycan binding proteins. Proc Natl Acad Sci USA 101:17033-17038PubMedCrossRefGoogle Scholar