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Production and Application of Glycan Microarrays

  • Julia Busch
  • Ryan McBride
  • Steven R. Head
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 632)

Abstract

Glycans are vital elements of living organisms and are involved in recognition, communication, cell growth and development, motility, and other significant processes. The interactions of glycans with the proteins that bind them provide valuable information about protein interaction and specificity. By printing glycans on microarrays, investigators are able to effectively determine the binding specificity of certain proteins with an extremely efficient and precise result. Such chips are performed by standard robotic microarray printing. Incubating the slides with various GBP-containing substances not only reveals clear receptor preferences of the proteins, but also detects minute differences in structure specificity.

Key words

Glycan Carbohydrate Lectin Microarray Glycan-binding protein Chip 

Notes

Acknowledgments

The authors would like to thank Ola Blixt, James Paulson, Nahid Razi, and Julia Hoffmann for all of their help and guidance. Andrew Hemingway and all of Schott/Nexterion for outstanding product support. Special thanks to the Consortium for Funtional Glycomics (CFG) for making everything possible.

References

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    Stevens J, Blixt O, Paulson JC, Wilson IA (2006) Glycan microarray technologies: tools to survey host specificity of influenza viruses. Nat Rev Microbiol 4:857-864PubMedCrossRefGoogle Scholar
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    Stevens J, Blixt O, Glaser L, Taubenberger JK, Palese P, Paulson JC, Wilson IA (2006) Glycan microarray analysis of the hemagglutinins from modern and pandemic influenza viruses reveals different receptor specificities. J Mol Biol 355:1143-1155PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.DNA Array Core FacilityThe Scripps Research InstituteLa JollaUSA

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