Multicenter Clinical Sample Collection for Microarray Analysis
In this chapter, we describe numerous methods to extract RNA, DNA, and protein from tissue, represented by kidney transplant biopsies, and from peripheral blood cells collected at various clinical sites. Gene expression profiling and SNP-based genome-wide association studies are done using various microarray platforms. In addition, protocols that enable simultaneous protein purification from these clinical samples, enable additional strategies for understanding of the molecular processes involved in organ transplantation, immunosuppressive drug regimens, and the elements determining allograft success and failure. Successfully establishing a multicenter clinical study was essential to meet our objectives for subject enrollment and transplant outcomes. This chapter focuses on our experience setting up and coordinating clinical sample collection from multiple transplant centers for the purpose of microarray analysis.
Key wordsMicroarrays Genomics Transplantation Multicenter clinical study Nucleic acid extraction Protein extraction
This work is supported by the National Institute of Allergy and Infectious Diseases (NIAID) Program Project Grant U19 AI63603 Genomics for Kidney Transplantation.
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