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An Efficient Protocol for VZV BAC-Based Mutagenesis

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In Vitro Mutagenesis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 634))

Abstract

Varicella-zoster virus (VZV) causes both varicella (chicken pox) and herpes zoster (shingles). As a member of the human herpesvirus family, VZV contains a large 125-kb DNA genome, encoding 70 unique open reading frames (ORFs). The genetic study of VZV has been hindered by the large size of viral genome, and thus the functions of the majority of these ORFs remain unclear. Recently, an efficient protocol has been developed based on a luciferase-containing VZV bacteria artificial chromosome (BAC) system to rapidly isolate and study VZV ORF deletion mutants.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey – New Jersey Medical School, Newark, NJ, USA

    Zhen Zhang, Ying Huang & Hua Zhu

Authors
  1. Zhen Zhang
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  2. Ying Huang
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  3. Hua Zhu
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Corresponding author

Correspondence to Hua Zhu .

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Editors and Affiliations

  1. Stratagene, North Torrey Pines Rd. 11011, La Jolla, 92037, USA

    Jeff Braman

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Zhang, Z., Huang, Y., Zhu, H. (2010). An Efficient Protocol for VZV BAC-Based Mutagenesis. In: Braman, J. (eds) In Vitro Mutagenesis Protocols. Methods in Molecular Biology, vol 634. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-652-8_5

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  • DOI: https://doi.org/10.1007/978-1-60761-652-8_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-651-1

  • Online ISBN: 978-1-60761-652-8

  • eBook Packages: Springer Protocols

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