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Use of Nanoparticles for Targeted, Noninvasive Thermal Destruction of Malignant Cells

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Cancer Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 624))

Abstract

Shortwave (MHz range) radiofrequency (RF) energy is nonionizing, penetrates deeply into biological tissues with no adverse side effects, and heats metallic nanoparticles efficiently. Targeted delivery of these nanoparticles to cancer cells should result in hyperthermic cytotoxicity upon exposure to a focused, noninvasive RF field. We have demonstrated that gold nanoparticles conjugated with cetuximab (C225) are quickly internalized by Panc-1 (pancreatic adenocarcinoma) and Difi (colorectal adenocarcinoma) cancer cells overexpressing epidermal growth factor receptor (EGFR). Panc-1 or Difi cells treated with naked gold nanoparticles or nonspecific IgG-conjugated gold nanoparticles demonstrated minimal intracellular uptake of gold nanoparticles by transmission electron microscopy (TEM). In contrast, there were dense concentrations of cytoplasmic vesicles containing gold nanoparticles following treatment with cetuximab-conjugated gold nanoparticles. Exposure of cells to a noninvasive RF field produced nearly 100% cytotoxicity in cells treated with the cetuximab-conjugated gold nanoparticles, but significantly lower levels of cytotoxicity in the two control groups (p < 0.00012). Treatment of a breast cancer cell line (CAMA-1) that does not express EGFR with cetuximab-conjugated gold nanoparticles produced no enhanced cytotoxicity following treatment in the RF field. Conjugation of cancer cell-directed targeting agents to gold nanoparticles may represent an effective and cancer-specific therapy to treat numerous types of human malignant disease using noninvasive RF hyperthermia.

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Author information

Authors and Affiliations

  1. Department of Surgical Oncology and Experimental Therapeutics, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA

    Paul Cherukuri

  2. Department of Chemistry, Smalley Institute for Nanoscale Science and Technology, Rice University, Houston, TX, USA

    Paul Cherukuri

  3. Department of Surgical Oncology, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA

    Steven A. Curley

  4. Mechanical Engineering and Material Science, Rice University, Houston, TX, USA

    Steven A. Curley

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  1. Paul Cherukuri
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  2. Steven A. Curley
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Editors and Affiliations

  1. Dept. Surgery, University of Florida, Gainesville, USA

    Stephen R. Grobmyer

  2. ERC - Engineering Research Center, University of Florida, Gainesville, USA

    Brij M. Moudgil

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Cherukuri, P., Curley, S.A. (2010). Use of Nanoparticles for Targeted, Noninvasive Thermal Destruction of Malignant Cells. In: Grobmyer, S., Moudgil, B. (eds) Cancer Nanotechnology. Methods in Molecular Biology, vol 624. Humana Press. https://doi.org/10.1007/978-1-60761-609-2_24

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  • DOI: https://doi.org/10.1007/978-1-60761-609-2_24

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