Synthesis of Antimicrobial Peptides Using the SPOT Technique

  • Dirk F. H. Winkler
  • Kai Hilpert
Part of the Methods in Molecular Biology book series (MIMB, volume 618)


Developing new lead structures for drugs against multiresistant bacteria is an urgent need for modern medicine. Antimicrobial peptides are a class of drugs that can be used to discover such structures. In order to support development of this research, a fast, easy, and inexpensive method to synthesize peptides is necessary. The SPOT synthesis has the potential to produce the required peptide arrays, synthesizing up to 8,000 peptides, peptide mixtures, or other organic compounds on cellulose or other planar surfaces in a positionally addressable and multiple manner. Protocols for the preparation of cellulose membranes and the SPOT synthesis as well as cleavage of peptides from the support are described.

Key words

SPOT synthesis peptide array screening antimicrobial peptides cellulose membranes 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Dirk F. H. Winkler
    • 1
  • Kai Hilpert
    • 2
  1. 1.Brain Research Centre, University of British ColumbiaVancouverCanada
  2. 2.Institute of Biological Interfaces – IBG 2, KIT (Karlsruhe Institute of Technology), Forschungszentrum KarlsruheEggenstein-LeopoldshafenGermany

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