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Bioinformatic Approaches to siRNA Selection and Optimization

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RNA Interference

Part of the book series: Methods in Molecular Biology ((MIMB,volume 623))

Abstract

RNA interference mediated by short interfering RNA (siRNA) molecules represents a powerful genetic tool with an increasing interest as potential therapeutics. Current bioinformatic approaches to design functional siRNA molecules take into account both empirical and rational approaches to identify selectable characteristics of active and specific siRNA molecules and focusing the downstream events in the RNAi pathway, such as target messenger RNA accessibility. The design of effective siRNA molecules is the key to successful experimentation with RNAi. Here, we show advanced siRNA design parameters and options for highly efficient siRNA candidate search.

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Authors and Affiliations

  1. Centre for BioAnalytical Sciences, Dublin City University, Dublin, Ireland

    Pirkko Muhonen & Harry Holthofer

Authors
  1. Pirkko Muhonen
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  2. Harry Holthofer
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Correspondence to Pirkko Muhonen .

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Editors and Affiliations

  1. Dept. Surgery, Pathology & Oncology, University of Western Ontario, Windermere Road 339, London, N6A 5A5, Canada

    Wei-Ping Min

  2. MediStem Laboratories, Inc., E. Cedar Street 2027, Tempe, 85281, USA

    Thomas Ichim

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Muhonen, P., Holthofer, H. (2010). Bioinformatic Approaches to siRNA Selection and Optimization. In: Min, WP., Ichim, T. (eds) RNA Interference. Methods in Molecular Biology, vol 623. Humana Press. https://doi.org/10.1007/978-1-60761-588-0_6

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  • DOI: https://doi.org/10.1007/978-1-60761-588-0_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-587-3

  • Online ISBN: 978-1-60761-588-0

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