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Using siRNA to Uncover Novel Oncogenic Signaling Pathways

  • Jin-Mei Lai
  • Chi-Ying F. Huang
  • Chang-Han Chen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 623)

Abstract

Tumor invasion and metastasis are the primary causes of cancer patient mortality, underscoring the need for identification of novel genes and signaling pathways that mediate these prognosis-determining phenomena. To identify and characterize novel lung adenocarcinoma genes associated with lung cancer progression, we created a bioinformatics-based approach that focuses on human cell-cycle-regulated genes that have evolved only in higher organisms but not in lower eukaryotic cells. In siRNA experiments in lung cancer cells, FLJ10540 was identified as one of several novel targets involved in cell migration and invasion. Here, we demonstrate that PI3K inhibition affects FLJ10540-mediated cell migration and invasion and further, that FLJ10540 knockdown ablates AKT-Ser473 phosphorylation. Taken together, these findings indicate that the FLJ10540/PI3K/AKT pathway may harbor new therapeutic targets for treating invasive lung adenocarcinoma.

Key words

FLJ10540 Migration Invasion PI3K/ AKT VEGF-A 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jin-Mei Lai
    • 1
  • Chi-Ying F. Huang
    • 2
  • Chang-Han Chen
    • 3
    • 4
  1. 1.Department of Life ScienceFu-Jen Catholic UniversityTaipeiTaiwan
  2. 2.Institute of Clinical MedicineNational Yang-Ming UniversityTaipeiTaiwan
  3. 3.Department of OtolaryngologyChang Gung MemorialHospital-Kaohsiung Medical Center, Chang Gung University College of MedicineKaohsiungTaiwan
  4. 4.Kaohsiung Chang Gung Head and Neck Oncology GroupChang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of MedicineKaohsiungTaiwan

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