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Statistical Methods for Proteomics

  • Klaus Jung
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 620)

Abstract

During the last decade, analytical methods for the detection and quantification of proteins and peptides in biological samples have been considerably improved. It is therefore now possible to compare simultaneously the expression levels of hundreds or thousands of proteins in different types of tissue, for example, normal and cancerous, or in different cell lines. In this chapter, we illustrate statistical designs for such proteomics experiments as well as methods for the analysis of resulting data. In particular, we focus on the preprocessing and analysis of protein expression levels recorded by the use of either two-dimensional gel electrophoresis or mass spectrometry.

Key words

Protein expression data preprocessing differential proteome analysis disease classification two-dimensional gel electrophoresis mass spectrometry 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Klaus Jung
    • 1
  1. 1.Department of Medical StatisticsGeorg-August-University GöttingenGöttingenGermany

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