Abstract
In this chapter, a multi-step protocol for covalently linking functionalized multi-walled carbon nanotubes (MWCNT) to deoxyribonucleic acid (DNA) oligonucleotides is provided. X-ray photoelectron spectroscopy (XPS) is used to characterize the initially formed amine-terminated MWCNTs, to which DNA is covalently anchored. Atomic force microscopy (AFM) investigation of the DNA-MWCNT conjugates reveals that the chemical functionalization occurs at both the ends and sidewalls of the nanotubes. The described methodology represents an important step toward the realization of DNA-guided self-assembly for carbon nanotubes.
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Acknowledgments
The financial support of Key Laboratory Funding Scheme of Shenzhen Municipal Government, BTC operation fund (CityU Project No. 9683001) and City University of Hong Kong (Project No. 7002100) are gratefully acknowledged.
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Chen, W., Yi, C., Chi-Hung, T., Lee, ST., Yang, M. (2010). Covalently Linked Deoxyribonucleic Acid with Multi-walled Carbon Nanotubes: Synthesis and Characterization. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_3
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DOI: https://doi.org/10.1007/978-1-60761-579-8_3
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