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Carbon Nanotube Biosensors with Aptamers as Molecular Recognition Elements

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Carbon Nanotubes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 625))

Abstract

In this chapter, we discuss in detail the fabrication of carbon nanotube biosensors that use a single-walled carbon nanotube field-effect transistor (SWNT-FET) as a transducer, and aptamers as molecular recognition elements. We use a patterned growth technique to grow SWNTs on Si/SiO2 substrates, and standard microfabrication procedures are then employed to fabricate sensing devices.

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References

  1. Patolsky F, Zheng G, Lieber CM (2006) Nanowire sensors for medicine and life sciences. Nanomedicine 1:51-65

    Article  CAS  PubMed  Google Scholar 

  2. Kong J, Franklin NR, Zhou C, Chaplin MG, Peng S, Cho K, Dai H (2000) Nanotube molecular wires as chemical sensors. Science 287:622-625

    Article  CAS  PubMed  Google Scholar 

  3. Chen RJ, Choi HC, Bangsaruntip S, Yenilmez E, Tang X, Wang Q, Chang Y-L, Dai H (2004) An investigations of the mechanisms of electronic sensing of protein adsorption on carbon nanotube devices. J Am Chem Soc 126:1563-1568

    Article  CAS  PubMed  Google Scholar 

  4. Besteman K, Lee J-O, Wiertz FGM, Heering HA, Dekker C (2003) Enzyme-coated carbon nanotubes as single molecule biosensors. Nano Lett 3:727-730

    Article  CAS  Google Scholar 

  5. So H-M, Won K, Kim YH, Kim B-K, Ryu BH, Na PS, Kim H, Lee J-O (2005) Carbon nanotube biosensors with aptamers as molecular recognition elements. J Am Chem Soc 127:11906-11907

    Article  CAS  PubMed  Google Scholar 

  6. So H-M, Park D-W, Jeon E-K, Kim Y-H, Kim BS, Lee C-K, Choi SY, Kim SC, Chang H, Lee J-O (2008) Detection and titer estimation of Escherichia coli using aptamer-functionalized single-walled carbon nanotube field effect transistors. Small 4:197-201

    Article  CAS  PubMed  Google Scholar 

  7. Lee J-O, So H-M, Jeon E-K, Chang H, Won K, Kim YH (2008) Aptamers as molecular recognition elements for electrical nanobiosensors. Anal Bioanal Chem 390:1023-1032

    Article  CAS  PubMed  Google Scholar 

  8. Lee JF, Stovall GM, Ellington AD (2006) Aptamer therapeutics advance. Curr Opin Chem Biol 10:282-289

    Article  CAS  PubMed  Google Scholar 

  9. Marciniak RA, Garcia-Blanco MA, Sharp PA (1990) Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus. Proc Natl Acad Sci USA 87:3624-3628

    Article  CAS  PubMed  Google Scholar 

  10. Maehashi K, Katsura T, Kerman K, Takamura Y, Matsumoto K (2007) Label free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors. Anal Chem 79:782-787

    Article  CAS  PubMed  Google Scholar 

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So, HM., Park, DW., Chang, H., Lee, JO. (2010). Carbon Nanotube Biosensors with Aptamers as Molecular Recognition Elements. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_20

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  • DOI: https://doi.org/10.1007/978-1-60761-579-8_20

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-577-4

  • Online ISBN: 978-1-60761-579-8

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