Abstract
A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 µg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wohlstadter JN, Wilbur JL, Sigal GB, Biebuyck HA, Billadeau MA, Dong LW, Fischer AB, Gudibande SR, Jamieson SH, Renten JH, Leginus J, Leland JK, Massey RJ, Wohlstadter SJ (2003) Carbon nanotube-based biosensor. Adv Mater 15:1184
Sánchez S, Pumera M, Fàbregas E (2007) Carbon nanotube/polysulfone screen-printed electrochemical immunosensor. Biosens Bioelectron 23:332
O’Connor M, Kim SN, Killard AJ, Forster RJ, Smyth MR, Papadimitrakopoulos F, Rusling JF (2004) Mediated amperometric immunosensing using single walled carbon nanotube forests. Analyst 129:1176
Yu X, Kim SN, Papadimitrakopoulos F, Rusling JF (2005) Protein immunosensor using single-wall carbon nanotube forests with electrochemical detection of enzyme labels. Mol Biosyst 1:70
Viswanathan S, Wu L-C, Huang M-R, Ho J-A (2006) Electrochemical immunosensor for cholera toxin using liposomes and poly(3, 4-ethylenedioxythiophene)-coated carbon nanotubes. Anal Chem 78:1115
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Sánchez, S., Fàbregas, E., Pumera, M. (2010). Detection of Biomarkers with Carbon Nanotube-Based Immunosensors. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_19
Download citation
DOI: https://doi.org/10.1007/978-1-60761-579-8_19
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-577-4
Online ISBN: 978-1-60761-579-8
eBook Packages: Springer Protocols