Abstract
We describe in detail the different steps involved in the construction of a carbon nanotube field-effect transistor (CNTFET) based on a network of single-walled carbon nanotubes (SWCNTs), which can selectively detect human immunoglobulin G (HIgG). HIgG antibodies, which are strongly adsorbed onto the walls of the SWCNTs, are the basic elements of the recognition layer. The nonspecific binding of proteins or other interferences are avoided by covering the nonadsorbed areas of the SWCNTs with Tween 20. The CNTFET is a reagentless device that does not need labels to detect HIgG.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Collins PG, Bradley K, Ishigami M, Zettl A (2000) Extreme oxygen sensitivity of electronic properties of carbon nanotubes. Science 287:1801-1804
Byon HR, Choi HC (2006) Network single-walled carbon nanotube-field effect transistors with increased schottky contact area for highly sensitive biosensor applications. J Am Chem Soc 128:2188-2189
Shim M, Shi-Kam NW, Chen RJ, Li Y, Dai H (2002) Functionalization of carbon nanotubes for biocompatibility and biomolecular recognition. Nano Lett 2:285-288
Star A, Gabriel JP, Bradley K, Grüner G (2003) Electronic detection of specific protein binding using nanotube FET devices. Nano Lett 3:459-463
So HM, Won K, Kim YH, Kim BK, Ryu BH, Na PS, Kim H, Lee JO (2005) Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements. J Am Chem Soc 127:11906-11907
Chen RJ, Bangsaruntip S, Drouvalakis KA, Kam NWS, Shim M, Li Y, Kim W, Utz PJ, Dai H (2003) Noncovalent functionalization of CNT for highly specific electronic biosensors. Proc Natl Acad Sci USA 100:4984-4989
Cid CC, Riu J, Maroto A, Rius FX (2008) Carbon nanotube field effect transistors for the fast and selective detection of human immunoglobulin G. Analyst 133:1005-1008
Maehashi K, Katsura T, Kerman K, Takamura Y, Matsumoto K, Tamiya E (2007) Label-free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors. Anal Chem 79:782-787
Star A, Tu E, Niemann J, Gabriel J-CP, Joiner CS, Valcke C (2006) Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors. Proc Natl Acad Sci USA 103:921-926
So H-M, Park D-W, Jeon E-K, Kim Y-H, Kim BS, Lee C-K, Choi SY, Kim SC, Chang H, Lee J-O (2008) Detection and titer estimation of Escherichia coli using aptamer-functionalized single-walled carbon-nanotube field-effect transistors. Small 4:197-201
Villamizar R, Maroto A, Rius FX, Inza I, Figueras MJ (2008) Fast detection of Salmonella Infantis with carbon nanotube field effect transistors Biosens Bioelectron 24:279-283
Sánchez-Acevedo Z, Riu J, Rius FX (2009) Fast picomolar selective detection of bisphenol A in water using a carbon nanotube field effect transistor functionalized with estrogen receptor-a Biosens Bioelectron 24:2842-2846
Kong J, Soh HT, Cassell AM, Quate CF, Dai H (1998) Synthesis of individual single-walled carbon nanotubes on patterned silicon wafers. Nature 395:878-881
Dai H (2002) Carbon nanotubes: synthesis, integration, and properties. Acc Chem Res 35:1035-1044
Su X, Chew FT, Li SFY (1999) Self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of total human immunoglobulin E. Anal Biochem 273:66-72
Veetil JV, Ye K (2007) Development of immunosensors using carbon nanotubes. Biotechnol Prog 23:517-531
Kojima A, Huon CK, Kamimura T, Maeda M, Matsumoto K (2005) Protein sensor using carbon nanotube field effect transistor. Jpn J Appl Phys 44:1596-1598
Hu L, Hecht DS, Grüner G (2004) Percolation in transparent and conducting carbon nanotube networks. Nano Lett 4:2513-2517
Martel R, Schmidt T, Shea HR, Hertel T, Avouris P (1998) Single- and multi-wall carbon nanotube field-effect transistors. Appl Phys Lett 73:2447-2449
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Cid, C.C., Riu, J., Maroto, A., Rius, F.X. (2010). Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_18
Download citation
DOI: https://doi.org/10.1007/978-1-60761-579-8_18
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-577-4
Online ISBN: 978-1-60761-579-8
eBook Packages: Springer Protocols