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Non-covalent Attachment of Proteins to Single-Walled Carbon Nanotubes

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Carbon Nanotubes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 625))

Abstract

A method for the non-covalent attachment of proteins to single-walled carbon nanotubes (SWNTs) is described. In this method, the protein is adsorbed to SWNTs that are suspended using sodium cholate, a surfactant and bile salt. The sodium cholate is then removed by dialysis with retention of the protein on the SWNTs. This method has resulted in good protein loadings and good retention of protein activity.

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Acknowledgments

This work was supported by the U.S. Department of Energy-Basic Energy Sciences (DE-FG02-06ER64239), the U.S. Department of Defense Breast Cancer Research Program (W81XWH-07-1-0536), and the Foundation for Science and Technology of Portugal (Luis Neves).

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Authors and Affiliations

  1. School of Chemical, Biological and Materials Engineering, and Carbon Nanotube Technology Center, University of Oklahoma, Norman, OK, USA

    Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan, David W. Schmidtke, Daniel E. Resasco & Roger G. Harrison

Authors
  1. Luís F. F. Neves
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  2. Ta-Wei Tsai
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  3. Naveen R. Palwai
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  4. David E. Martyn
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  5. Yongqiang Tan
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  6. David W. Schmidtke
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  7. Daniel E. Resasco
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  8. Roger G. Harrison
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Editor information

Editors and Affiliations

  1. MPI für Festkörperforschung, Heisenbergstr. 1, Stuttgart, 70569, Germany

    Kannan Balasubramanian

  2. MPI für Festkörperforschung, Heisenbergstr. 1, Stuttgart, 70569, Germany

    Marko Burghard

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Neves, L.F.F. et al. (2010). Non-covalent Attachment of Proteins to Single-Walled Carbon Nanotubes. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_1

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  • DOI: https://doi.org/10.1007/978-1-60761-579-8_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-577-4

  • Online ISBN: 978-1-60761-579-8

  • eBook Packages: Springer Protocols

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