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Peptidomics pp 57–73Cite as

Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 615))

Abstract

Neuropeptides are important signaling molecules that regulate many essential physiological processes. Microdialysis offers a way to sample neuropeptides in vivo. When combined with liquid chromatography–mass spectrometry detection, many known and unknown neuropeptides can be identified from a live organism. This chapter describes sample preparation techniques and general strategies for the mass spectral analysis of neuropeptides collected via microdialysis sampling. Methods for the in vitro microdialysis of a neuropeptide standard as well as the in vivo microdialysis sampling of neuropeptides from a live crab are described.

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Acknowledgments

The authors wish to thank Professor Craig Berridge (Department of Psychology, University of Wisconsin-Madison) for helpful discussions about microdialysis. This work was supported in part by the School of Pharmacy and the Wisconsin Alumni Research Foundation at the University of Wisconsin-Madison, a National Science Foundation CAREER Award (CHE-0449991), and the National Institutes of Health through Grant 1R01DK071801. L.L. acknowledges an Alfred P. Sloan Research Fellowship. H.L.B. acknowledges the National Institutes of Health Biotechnology Training Grant 5T32 GM08349.

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Authors and Affiliations

  1. Department of Chemistry & School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA

    Heidi L. Behrens

  2. Department of Chemistry, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA

    Lingjun Li

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  1. Heidi L. Behrens
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  2. Lingjun Li
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Correspondence to Heidi L. Behrens .

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Editors and Affiliations

  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

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Behrens, H.L., Li, L. (2010). Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry. In: Soloviev, M. (eds) Peptidomics. Methods in Molecular Biology, vol 615. Humana Press. https://doi.org/10.1007/978-1-60761-535-4_5

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  • DOI: https://doi.org/10.1007/978-1-60761-535-4_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-534-7

  • Online ISBN: 978-1-60761-535-4

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