Abstract
Here we report our approach to the peptidomic analysis of mouse liver. We use ultrafiltration for peptide prefractionation, which is followed by size exclusion chromatography. The low molecular weight peptides (MW below ~3 kDa) are analysed directly by nanoLC-MS/MS, and the higher molecular weight peptides (MW above ~3 kDa) are characterized with MALDI-TOF MS first and then proteolytically digested prior to nanoLC-MS/MS analyses.
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Acknowledgments
This work was supported by National Natural Sciences Foundation of China (No. 20735004), the China High Technology Research Program Grant (2006AA02A309) and the Knowledge Innovation program of DICP to H. Zou.
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Hu, L., Ye, M., Zou, H. (2010). Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation. In: Soloviev, M. (eds) Peptidomics. Methods in Molecular Biology, vol 615. Humana Press. https://doi.org/10.1007/978-1-60761-535-4_16
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DOI: https://doi.org/10.1007/978-1-60761-535-4_16
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