Abstract
Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to analyze the peptide complement in the nervous system of a variety of invertebrate animals from leech to Aplysia and many arthropod species, especially insects and crustaceans. Here, we describe a protocol for direct peptide profiling of defined areas of the central nervous system of insects. With this method, one can routinely and reliably obtain neuropeptide signatures of selected brain areas from various insects.
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References
Predel, R., Neupert, S., Roth, S., Derst, C., and Nässel, D. (2005) Tachykinin-related peptide precursors in two cockroach species: molecular cloning and peptide expression. FEBS J. 272, 3365–3375.
Schachtner, J., Schmidt, M., and Homberg, U. (2005) Organization and evolutionary trends of primary olfactory brain centers in Tetraconata (Crustacea Hexapoda). Arthropod Struct. Dev. 34, 257–299.
Utz, S., Huetteroth, W., Predel, R., Wegener, C., Kahnt, J., and Schachtner, J. (2007) Direct peptide profiling of lateral cell groups of the antennal lobes of Manduca sexta reveals specific composition and changes in neuropeptide expression during development. Dev. Neurobiol. 67, 764–777.
Utz, S., Huetteroth, W., Vömel, M., and Schachtner, J. (2008) Mas-allatotropin in the developing antennal lobe of the sphinx moth Manduca sexta: Distribution, time course, developmental regulation and colocalization with other neuropeptides. Dev. Neurobiol. 68, 123–142.
Weevers, R.D. (1966) A lepidopteran saline: the effects of inorganic cation concentrations on sensory reflex and motor responses in a herbivorous insect. J. Exp. Biol. 44, 163–176.
Southey, B.R., Amare, A., Zimmerman, T.A., Rodriguez-Zas, S.L., and Sweedler, J.V. (2006) NeuroPred: a tool to predict cleavage sites in neuropeptide precursors and provide the masses of the resulting peptides. Nucleic Acids Res. 34, 267–272.
Berg, B.G., Schachtner, J., Utz, S., and Homberg, U. (2007) Distribution of neuropeptides in the primary olfactory centre of the heliothine moth Heliothis virescens. Cell Tissue Res. 327, 385–398.
Li, B., Predel, R., Neupert, S., Hauser, F., Tanaka, Y., Verleyen, P., Cazzamali, G., Williamson, M., Schoofs, L., Schachtner, J., Grimmelikhuijzen, C., and Park, Y. (2008) Genomics, transcriptomics, peptidomics, and evolution of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum. Genome Res. 18, 113–122.
Siviter, R.J., Coast, G.M., Winther, Å.M.E., Nachman, R.J., Taylor, C.A.M., Shirras, A.D., Coates, D., Isaac, R.E., and Nässel, D.R. (2000) Expression and functional characterization of a Drosophila neuropeptide precursor with homology to mammalian preprotachykinin A. J. Biol. Chem. 275, 23273–23280.
Winther Å.M.E., Siviter R.J., Isaac R.E., Predel R., Nässel D.R. (2003). Neuronal expression of tachykinin-related peptides and gene transcript during postembryonic development of Drosophila. J. Comp. Neurol. 464:180–196.
Acknowledgments
Original work was supported by the Deutsche Forschungsgemeinschaft (DFG, SCHA 678/3-3; PR 766/9-1 to R. Predel, J. Schachtner, and C. Wegener; WE 2652/2) and Peter und Traudl Engelhorn Stiftung (to SN). We like to thank William K. Russell (College Station, TX), Jörg Kahnt, and Lotte Søgaard-Andersen (Max-Planck-Institute of Terrestrial Microbiology, Marburg) for the use of the mass spectrometer and Sandra Utz (Marburg, Germany) for supporting this study.
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Schachtner, J., Wegener, C., Neupert, S., Predel, R. (2010). Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry. In: Soloviev, M. (eds) Peptidomics. Methods in Molecular Biology, vol 615. Humana Press. https://doi.org/10.1007/978-1-60761-535-4_10
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DOI: https://doi.org/10.1007/978-1-60761-535-4_10
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