Abstract
Lentiviral vectors are a powerful tool to achieve regulated expression of transgenes in vivo and in vitro. The doxycycline-inducible system is well characterized and can be used to regulate expression mediated by lentiviral vectors. Because many different doxycycline-inducible lentiviral vectors have been described, choosing the best vector system can be difficult. This chapter can be used as a guide to select the optimal system for a particular application.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Clackson T. (2000) Regulated gene expression systems. Gene Ther 7,120-5.
Gossen M, and Bujard H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A 89, 5547-51.
Agha-Mohammadi S, and Lotze MT. (2000) Regulatable systems: applications in gene therapy and replicating viruses. J Clin Invest 105, 1177-83.
Corbel SY, and Rossi FM. (2002) Latest developments and in vivo use of the Tet system: ex vivo and in vivo delivery of tetracycline-regulated genes. Curr Opin Biotechnol 13, 448-52.
Goverdhana S, Puntel M, Xiong W et al. (2005) Regulatable gene expression systems for gene therapy applications: progress and future challenges. Mol Ther 12, 189-211.
Toniatti C, Bujard H, Cortese R, and Ciliberto G. (2004) Gene therapy progress and prospects: transcription regulatory systems. Gene Ther 11, 649-57.
Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, and Bujard H. (1995) Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766-69.
Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, and Hillen W. (2000) Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci U S A 97, 7963-8.
Zhou X, Vink M, Klaver B, Berkhout B, and Das AT. (2006) Optimization of the Tet-On system for regulated gene expression through viral evolution. Gene Ther 13, 1382-90.
Freundlieb S, Schirra-Muller C, and Bujard H. (1999). A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells. J Gene Med 1, 4-12.
Szulc J, Wiznerowicz M, Sauvain MO, Trono D, and Aebischer P. (2006). A versatile tool for conditional gene expression and knockdown. Nat Methods 3, 109-16.
Markusic D, Oude-Elferink R, Das AT, Berkhout B, and Seppen J. (2005) Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter. Nucleic Acids Res 33, e63.
Wiznerowicz M, Szulc J, and Trono D. (2006) Tuning silence: conditional systems for RNA interference. Nat Methods 3, 682-8.
Zeng Y, Wagner EJ, and Cullen BR. (2002) Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol Cell 9, 1327-33.
Ginhoux F, Turbant S, Gross DA et al. (2004) HLA-A*0201-restricted cytolytic responses to the rtTA transactivator dominant and cryptic epitopes compromise transgene expression induced by the tetracycline on system. Mol Ther 10, 279-89.
Latta-Mahieu M, Rolland M, Caillet C et al. (2002) Gene transfer of a chimeric trans-activator is immunogenic and results in short-lived transgene expression. Hum Gene Ther 13, 1611-20.
Hojman P, Eriksen J, and Gehl J. (2007) Tet-On induction with doxycycline after gene transfer in mice: sweetening of drinking water is not a good idea. Anim Biotechnol 18, 183-8.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Markusic, D., Seppen, J. (2010). Doxycycline Regulated Lentiviral Vectors. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology, vol 614. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-533-0_4
Download citation
DOI: https://doi.org/10.1007/978-1-60761-533-0_4
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-532-3
Online ISBN: 978-1-60761-533-0
eBook Packages: Springer Protocols