Abstract
Phencyclidine (PCP) is a cycloalkylamine and is classified as a dissociative anesthetic. In the1950s, PCP was tested as an intravenous anesthetic but due to its severe side effects, it was withdrawn from the clinical use. Since then PCP has become an illegal street drug making its laboratory analysis forensically essential. PCP can be detected in urine, serum, or plasma by immunoassays and quantified by gas or liquid chromatography mass spectrometry. In the method described here, a deuterated internal standard is added to the sample and the drug is extracted under alkaline conditions. Analysis is conducted using gas chromatography mass spectrometry (GC-MS). Quantitation of PCP is done by comparing the responses of unknown samples to the standards using selected ion monitoring.
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References
Baselt, R.C., Phencyclidine, in Disposition of Toxic Drugs and Chemicals in Man. 2002, Biomedical Publications: Foster City, pp. 827–830.
Fenton, J.J., Drugs of Abuse, in Toxicology: A Case-oriented Approach. 2002, CRC Press: Boca Raton, pp. 359–401.
Porter, W.H., Clinical Toxicology, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, C.A. Burtis, E.R. Ashwood, and D.E. Bruns, Editors. 2006, Elsevier Saunders: St. Louis, MO, pp. 1287–1369.
Kwong, T.C., Introduction to Drug of Abuse Testing, in Handbook of Drug Monitoring Methods: Therapeutics and Drugs of Abuse, A. Dasgupta, Editor. 2008, Humana Press: Totowa, NJ, pp. 297–315.
Boeckx, R., False positive EMIT DAU PCP assay as a result of an overdose of dextromethorphan. Clin Chem, 1987, 33: 974–975.
Levine, B. and M. Smith, Effects of diphenhydramine on immunoassay of phencyclidine in urine. Clin Chem, 1990, 36: 1258.
Long, C., J. Crifasi, and D. Maginn, Interference of thioridazine (Mellaril) in identification of phencyclidine. Clin Chem, 1996, 42: 1885–1886.
Kala, S.V., et al., Validation of analysis of amphetamines, opiates, phencyclidine, cocaine, and benzoylecgonine in oral fluids by liquid chromatography-tandem mass spectrometry. J Anal Toxicol, 2008, 32(8): 605–611.
Coulter, C., K. Crompton, and C. Moore, Detection of phencyclidine in human oral fluid using solid-phase extraction and liquid chromatography with tandem mass spectrometric detection. J Chromatogr B Analyt Technol Biomed Life Sci, 2008, 863(1): 123–128.
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Ferguson, A.M., Garg, U. (2010). Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS). In: Garg, U., Hammett-Stabler, C. (eds) Clinical Applications of Mass Spectrometry. Methods in Molecular Biology, vol 603. Humana Press. https://doi.org/10.1007/978-1-60761-459-3_45
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DOI: https://doi.org/10.1007/978-1-60761-459-3_45
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