Abstract
A detailed protocol using liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) is described for the determination of the total homocysteine (HCY) in plasma. Sample preparation is simple and relies on the complete reduction of any homocystine ((HCY)2) or protein-bound homocysteine, followed by the plasma protein precipitation. Any factor influencing the measurement is addressed. The protocol is robust and suitable for large-scale routine determinations.
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References
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© 2010 Humana Press, a part of Springer Science+Business Media, LLC
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Casetta, B. (2010). Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC/MS/MS). In: Garg, U., Hammett-Stabler, C. (eds) Clinical Applications of Mass Spectrometry. Methods in Molecular Biology, vol 603. Humana Press. https://doi.org/10.1007/978-1-60761-459-3_23
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DOI: https://doi.org/10.1007/978-1-60761-459-3_23
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Online ISBN: 978-1-60761-459-3
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