Abstract
Centrifuged urine, internal standard (promazine), and ammonium formate buffer are mixed in an autosampler vial to achieve a 10-fold dilution of the specimen. Without additional pretreatment, 10 µL of the sample is injected onto a C18 reverse phase column for gradient analysis with ammonium formate/acetonitrile mobile phases. Drugs in the column eluent become charged in the ion source using positive electrospray atmospheric pressure ionization. Pseudomolecular drug ions are analyzed by a hybrid triple quadrupole linear ion trap mass spectrometer operated with a 264-drug selected ion monitoring (SRM) acquisition method that includes an information-dependant acquisition (IDA) algorithm.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mueller CA, Weinmann W, Dresen S, Schreiber A, Gergov M. (2005) Development of a multi-target screening analysis for 301 drugs using a QTrap liquid chromatography/tandem mass spectrometry system and automated library searching. Rapid Commun Mass Spectrom 19, 1332–1338.
Maurer HH. (2006) Hyphenated mass spectrometric techniques-indispensable tools in clinical and forensic toxicology and in doping control. J Mass Spectrom 4, 1399–1413. Review.
Marquet P. (2002) Is LC-MS suitable for a comprehensive screening of drugs and poisons in clinical toxicology? Ther Drug Monit 24, 125–133. Review.
Saint-Marcoux F, Lachâtre G, Marquet P. (2003) Evaluation of an improved general unknown screening procedure using liquid chromatography-electrospray-mass spectrometry by comparison with gas chromatography and high-performance liquid-chromatography–diode array detection. J Am Soc Mass Spectrom 14, 14–22.
Sauvage FL, Saint-Marcoux F, Duretz B, Deporte D, Lachâtre G, Marquet P. (2006) Screening of drugs and toxic compounds with liquid chromatography-linear ion trap tandem mass spectrometry. Clin Chem 52, 1735–1742.
Lynch K, et al. Performance evaluation of three liquid chromatograph-mass spectrometry methods for broad spectrun drug screening. Clin Chem Submitted (July 2009).
Chase D et al. (2007) Mass Spectrometry in the Clinical Laboratory: General Principles and Guidance; Approved Guideline C50-A. Clinical and Laboratory Standards Institute, Wayne, PA.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Stone, J. (2010). Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole Linear Ion Trap Mass Spectrometry. In: Garg, U., Hammett-Stabler, C. (eds) Clinical Applications of Mass Spectrometry. Methods in Molecular Biology, vol 603. Humana Press. https://doi.org/10.1007/978-1-60761-459-3_19
Download citation
DOI: https://doi.org/10.1007/978-1-60761-459-3_19
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-458-6
Online ISBN: 978-1-60761-459-3
eBook Packages: Springer Protocols