Abstract
Systematic studying of biological processes driven by multipoint high-cooperative carbohydrate recognition requires application of multivalent carbohydrates as tools. In this regard polyacrylamides with various pendant carbohydrate residues and labels are probably the most well advanced class of carbohydrate multimerics. Here we describe a synthetic approach to polyacrylamide-based glycoconjugates with biotin tag, with special emphasis to development of carbohydrate biosensors and arrays.
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Abbreviations
- AC:
-
6-aminocaproyl
- AIBN:
-
α,α ′-azoisobutyronitrile
- biot:
-
biotin
- Btri :
-
Galα1-3(Fucα1-2)Gal-
- DP:
-
average degree of polymerization
- ELISA:
-
enzyme-linked immunosorbent assay
- Glyc:
-
glycoside residue
- GPC:
-
gel-permeation chromatography
- M n :
-
number average molecular mass
- M w :
-
weight average molecular mass
- NPA:
-
4-nitrophenylacrylate
- pHEAA:
-
poly(N-(2-hydroxyethyl)acrylamide)
- pNPA:
-
poly(4-nitrophenylacrylate)
- Str:
-
streptavidin.
References
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Acknowledgments
This work was supported by the RAS Presidium Program “Molecular and cell biology” and RFBR grant # 07-04-00630.
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Chinarev, A.A., Galanina, O.E., Bovin, N.V. (2010). Biotinylated Multivalent Glycoconjugates for Surface Coating. In: Li, J. (eds) Functional Glycomics. Methods in Molecular Biology, vol 600. Humana Press. https://doi.org/10.1007/978-1-60761-454-8_5
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DOI: https://doi.org/10.1007/978-1-60761-454-8_5
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