Abstract
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
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Acknowledgement
The work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan.
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© 2010 Humana Press, a part of Springer Science+Business Media, LLC
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Takei, K., Yamada, H., Abe, T. (2010). Use of Liposomes to Study Vesicular Transport. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology™, vol 606. Humana Press. https://doi.org/10.1007/978-1-60761-447-0_36
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DOI: https://doi.org/10.1007/978-1-60761-447-0_36
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