Abstract
The intracellular distribution of nanoparticular drug delivery systems is very complex, but its investigation yields high potential for further development and optimization of these systems.
In the following chapter, we introduce the application of fluorescent imaging techniques in order to highlight uptake and cellular processing of nanoparticular drug delivery systems (e.g., liposomal drug delivery systems). We selected a combination of different protocols for the staining of the most important endocytic compartments and organelles. The presented imaging systems are appropriate to detect liposomal drug delivery systems localized in these cellular structures.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
van der Aa MA, Huth US, Hafele SY et al (2007) Cellular uptake of cationic polymer-DNA complexes via caveolae plays a pivotal role in gene transfection in COS-7 cells. Pharm Res 24(8):1590-1598
Huth US, Schubert R, Peschka-Suss R (2006) Investigating the uptake and intracellular fate of pH-sensitive liposomes by flow cytometry and spectral bio-imaging. J Control Release 110(3):490-504
Huth U, Wieschollek A, Garini Y, Schubert R, Peschka-Suss R (2004) Fourier transformed spectral bio-imaging for studying the intracellular fate of liposomes. Cytometry A 57(1):10-21
Karin M, Mintz B (1981) Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells. J Biol Chem 256(7):3245-3252
Chinnapen DJ, Chinnapen H, Saslowsky D, Lencer WI (2007) Rafting with cholera toxin: endocytosis and trafficking from plasma membrane to ER. FEMS Microbiol Lett 266(2):129-137
Singh RD, Puri V, Valiyaveettil JT, Marks DL, Bittman R, Pagano RE (2003) Selective caveolin-1-dependent endocytosis of glycosphingolipids. Mol Biol Cell 14(8):3254-3265
Mu FT, Callaghan JM, Steele-Mortimer O et al (1995) EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine “fingers” and contains a calmodulin-binding IQ motif. J Biol Chem 270(22):13503-13511
Chen JW, Murphy TL, Willingham MC, Pastan I, August JT (1985) Identification of two lysosomal membrane glycoproteins. J Cell Biol 101(1):85-95
Bush WS, Ihrke G, Robinson JM, Kenworthy AK (2006) Antibody-specific detection of caveolin-1 in subapical compartments of MDCK cells. Histochem Cell Biol 126(1):27-34
Pelkmans L, Kartenbeck J, Helenius A (2001) Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER. Nat Cell Biol 3(5):473-483
Le PU, Nabi IR (2003) Distinct caveolae-mediated endocytic pathways target the Golgi apparatus and the endoplasmic reticulum. J Cell Sci 116(Pt 6):1059-1071
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Schneider, S., Süss, R. (2010). Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology™, vol 606. Humana Press. https://doi.org/10.1007/978-1-60761-447-0_31
Download citation
DOI: https://doi.org/10.1007/978-1-60761-447-0_31
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-446-3
Online ISBN: 978-1-60761-447-0
eBook Packages: Springer Protocols