Abstract
Atomic Force Microscopy (AFM) is a useful tool for the visualization of soft biological samples in a nanoscale resolution. In the study presented here, the surface morphology ofP-selectin and Transferrin modified proteoliposomes were investigated in air and under water. The proteins were visualized without pre-functionalization or staining.
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References
Lukas K, Zhifeng S (1998) The application of AFM to biomembranes Biomembrane Structures
Schneider S, Lärmer J, Henderson R, Oberleithner H (1998) Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopy. Pflügers Arch 435:362-7
Rasch P, Wiedemann U, Wienberg J, Heckl W (1993) Analysis of banded human chromosomes and in situ hybridization patterns by scanning force microscopy. Proc Natl Acad Sci USA 90:2509
Hansma HG, Kasuya K, Oroudjev E (2004) Atomic force microscopy imaging and pulling of nucleic acids. Curr Opin Struct Biol 14:380-5
Kamruzzahan A, Kienberger F, Stroh C et al (2004) Imaging morphological details and pathological differences of red blood cells using tapping-mode. AFM Biol Chem 385:955-60
Engel A, Müller D (2000) Observing single biomolecules at work with the atomic force microscope. Nat Struct Biol 7:715-8
Müller DJ, Engel A (1999) Voltage and pH-induced channel closure of porin OmpF visualized by atomic force microscopy. J Mol Biol 285:1347-51
Zeidel M, Nielsen S, Smith B, Ambudkar S, Maunsbach A, Agre P (1994) Ultrastructure, pharmacologic inhibition, and transport selectivity of aquaporin channel-forming integral protein in proteoliposomes. Biochemistry 33:1606-15
Awasthi S, Singhal S, Pikula S et al (1998) ATP-Dependent human erythrocyte glutathione-conjugate transporter. II. Functional reconstitution of transport activity. Biochemistry 37:5239-48
Dass C (2008) Drug delivery in cancer using liposomes. Methods Mol Biol 437:177-82
Lian T, Ho R (2001) Trends and developments in liposome drug delivery systems Journal of Pharmaceutical. Sciences 90:667-80
Opinion E (2008) Antibody-targeted liposomes in cancer therapy and imaging. Expert Opin Drug Deliv 5:189-204
Bendas G, Krause A, Bakowsky U, Vogel J, Rothe U (1999) Targetability of novel immunoliposomes prepared by a new antibody conjugation technique. Int J Pharm 181:79-93
Pignataro B, Steinem C, Galla H, Fuchs H, Janshoff A (2000) Specific adhesion of vesicles monitored by scanning force microscopy and quartz crystal microbalance. Biophys J 78:487-98
Moore K (1991) GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction. J Cell Biol 112:491-9
Rigaud J (2002) Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals Brazilian. J Med Biol Res 35:753-66
Anabousi S, Laue M, Lehr C, Bakowsky U, Ehrhardt C (2005) Assessing transferrin modification of liposomes by atomic force microscopy and transmission electron microscopy. Eur J Pharm Biopharm 60:295-303
Oberle V, Bakowsky U, Zuhorn I, Hoekstra D (2000) Lipoplex formation under equilibrium conditions reveals a three-step mechanism. Biophys J 79:1447-54
Kneuer C, Ehrhardt C, Bakowsky H et al (2006) The influence of physicochemical parameters on the efficacy of non-viral DNA transfection complexes: a comparative study. Journal of Nanoscience and Nanotechnology 6:2776-82
Acknowledgments
This work was supported in parts by DFG Forschergruppe 627 Nanohale and JPK Instruments Berlin (Germany) (UB and JS). This work was supported in part by grants from Enterprise Ireland under the National Development Plan co-funded by EU Structural Funds and Science Foundation Ireland (CE and MMG).
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Sitterberg, J., Gaspar, M.M., Ehrhardt, C., Bakowsky, U. (2010). Atomic Force Microscopy for the Characterization of Proteoliposomes. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology™, vol 606. Humana Press. https://doi.org/10.1007/978-1-60761-447-0_23
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DOI: https://doi.org/10.1007/978-1-60761-447-0_23
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