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Atomic Force Microscopy for the Characterization of Proteoliposomes

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Book cover Liposomes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 606))

Abstract

Atomic Force Microscopy (AFM) is a useful tool for the visualization of soft biological samples in a nanoscale resolution. In the study presented here, the surface morphology ofP-selectin and Transferrin modified proteoliposomes were investigated in air and under water. The proteins were visualized without pre-functionalization or staining.

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Acknowledgments

This work was supported in parts by DFG Forschergruppe 627 Nanohale and JPK Instruments Berlin (Germany) (UB and JS). This work was supported in part by grants from Enterprise Ireland under the National Development Plan co-funded by EU Structural Funds and Science Foundation Ireland (CE and MMG).

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Technology and Biopharmacy, Philipps-Universität Marburg, Marburg, Germany

    Johannes Sitterberg

  2. Unidade Novas Formas de Agentes Bioactivos, iMed, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal

    Maria Manuela Gaspar

  3. School of Pharmacy and Pharmaceutical Sciences, University of Dublin, Trinity College Dublin, Dublin, Ireland

    Carsten Ehrhardt

  4. Department of Pharmaceutical Technology and Biopharmacy, Philipps-Universität Marburg, Marburg, Germany

    Udo Bakowsky

Authors
  1. Johannes Sitterberg
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  2. Maria Manuela Gaspar
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  3. Carsten Ehrhardt
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  4. Udo Bakowsky
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Editor information

Editors and Affiliations

  1. College of Pharmacy, Midwestern University, N. 59th Avenue 19555, Glendale, 85308, U.S.A.

    Volkmar Weissig

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Sitterberg, J., Gaspar, M.M., Ehrhardt, C., Bakowsky, U. (2010). Atomic Force Microscopy for the Characterization of Proteoliposomes. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology™, vol 606. Humana Press. https://doi.org/10.1007/978-1-60761-447-0_23

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  • DOI: https://doi.org/10.1007/978-1-60761-447-0_23

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-446-3

  • Online ISBN: 978-1-60761-447-0

  • eBook Packages: Springer Protocols

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