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Dendritic Cell Protocols pp 117–133Cite as

Generation of Stable Th1/CTL-, Th2-, and Th17-Inducing Human Dendritic Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 595))

Abstract

Dendritic cells (DC) are the most potent inducers and regulators of immune responses, responsible for communication within immune system. The ability of DC to act both as the inducers of immune responses and as regulatory/suppressive cells led to the interest in their immunotherapeutic use in different disease types, ranging from cancer to autoimmunity, and as a tool to prevent the rejection of transplanted tissues and organs. Over the last years, several groups including ours have demonstrated the feasibility of obtaining monocyte-derived DC with different functions, by modulating the conditions and the duration of DC maturation. The current chapter provides a detailed protocol of generating type-1-, type-2-, and type-17-polarized DC for testing the cytokine-producing abilities of these cells and their effectiveness in inducing Th1, Th2, and Th17 responses of CD4+ T cells and CTL responses of naïve and memory CD8+ T cells.

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Acknowledgments

This work was supported by the NCI grants CA95128, CA101944, and CA114931.

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Authors and Affiliations

  1. Departments of Surgery, Immunology, and Infectious Diseases and Microbiology, University of Pittsburgh and University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA

    Pawel Kalinski, Eva Wieckowski & Ravikumar Muthuswamy

  2. Department of Cell Biology and Immunology, Academic Medical Center, Amsterdam, The Netherlands

    Esther de Jong

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  1. Pawel Kalinski
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  2. Eva Wieckowski
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  4. Esther de Jong
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  1. Quellijnstraat 25A, Amsterdam, 1072 XN, Netherlands

    Shalin H. Naik

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Kalinski, P., Wieckowski, E., Muthuswamy, R., de Jong, E. (2010). Generation of Stable Th1/CTL-, Th2-, and Th17-Inducing Human Dendritic Cells. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_7

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