Abstract
Naturally occurring thymus-derived CD4+CD25+Foxp3+ regulatory T cells (nTregs) are critical regulators of immune tolerance. Foxp3+ Tregs can also be induced from CD25− naïve CD4 T cells in vivo and ex vivo. This conversion process requires cytokines such as IL2 and TGFβ as well as suboptimal TCR stimulation, thus is regulated by the co-stimulatory status of antigen-presenting cells (i.e., dendritic cells). Although mature dendritic cells (DCs) are potent initiators of adaptive immune response, immature steady-state DCs contribute to immune tolerance. In this chapter, we summarize methods that use ex vivo splenic DCs to induce the conversion of naïve CD4+ T cells to adaptive Foxp3+CD4+ regulatory T cells (aTreg) in the presence of TGFβ.
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Wang, L. (2010). Adaptive Treg Generation by DCs and Their Functional Analysis. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_26
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DOI: https://doi.org/10.1007/978-1-60761-421-0_26
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