Abstract
Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes without the induction of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.
Key words
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- BAL:
-
bronchoalveolar lavage
- DC:
-
dendritic cell
- MLN:
-
mediastinal lymph node
- IN:
-
Intranasal
- IT:
-
Intratracheal
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Acknowledgments
This work was supported by NIH grant AI49653 to GJR, an institutional training grant to CJ, and a Primary Caregiver Supplement to CJ by NIAID. GJR is an established investigator of the American Heart Association.
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Jakubzick, C., Randolph, G.J. (2010). Methods to Study Pulmonary Dendritic Cell Migration. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_24
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DOI: https://doi.org/10.1007/978-1-60761-421-0_24
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