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Isolation of Cutaneous Dendritic Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 595))

Abstract

Cutaneous dendritic cells represent the first immunological interface with the environment and play a key role in the defense against pathogens that breach the skin. This protocol describes how to isolate cutaneous dendritic cells from mouse ears for flow cytometry analysis and functional assay studies.

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References

  1. Merad, M., Ginhoux, F. & Collin, M. Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cells. Nat Rev Immunol 8, 935–947 (2008).

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  2. Ginhoux, F., et al. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204, 3133–3146 (2007).

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  3. Poulin, L.F., et al. The dermis contains langerin+ dendritic cells that develop and function independently of epidermal Langerhans cells. J Exp Med 204, 3119–3131 (2007).

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  4. Bursch, L.S., et al. Identification of a novel population of Langerin+ dendritic cells. J Exp Med 204, 3147–3156 (2007).

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Helft, J., Merad, M. (2010). Isolation of Cutaneous Dendritic Cells. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_15

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  • DOI: https://doi.org/10.1007/978-1-60761-421-0_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-420-3

  • Online ISBN: 978-1-60761-421-0

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