Abstract
The CD8+, CD8−, and plasmacytoid dendritic cell (DC) subtypes develop from progenitors that express surface fms-like tyrosine kinase 3 (Flt3). Recently, two developmentally sequential progenitors have been identified that give rise to these subtypes. This includes a transition from an earlier CD11c−MHC-II− “pro-DC,” which divides and differentiates to give rise to CD11c+MHC-II− “pre-DC,” en route to generating the three CD11c+MHC-II+ DC subtypes – plasmacytoid DCs, CD8+ DCs, and CD8− DCs. In this chapter, we describe the very simple method of generating large numbers of in vitro-derived pro-DCs and pre-DCs. As these precursors are largely DC-restricted, they can be used to either reconstitute a mouse with DCs of desired background, to study the developmental steps in vitro or in vivo, among other purposes.
Key words
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Naik, S.H. (2010). Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_11
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DOI: https://doi.org/10.1007/978-1-60761-421-0_11
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